Services
Mass Spectrometry Imaging produces the spatial distribution of various analyte classes present in tissue samples mounted on microscope slides. By bringing MSI together with other imaging modalities such as microscopy and MRI, a more holistic picture of biomolecular imaging can be achieved. Within the facility we have a number of MALDI-TOF/TOF systems, and an atmospheric pressure MALDI which can be coupled to QqTOF and QIT analysers. We also now have a JEOL MALDI Spiral-TOF, capable of higher mass resolving power (75,000). Recent addition is a Bruker TIMS TOF Flex with MALDI-2, an instrument capable of both MALDI and Electrospray, making it a very useful instrument for Mass Spectrometry Imaging and companion LC-MS analysis. The broad range of instruments allow us to perform Mass Spectrometry Imaging for a diverse range of analytes. In addition to mass spectrometers, we have a suite of supporting equipment including HPLC-spotting robots, and matrix deposition devices (both spray and vacuum sublimation based. A range of supporting software is also available, most of which can be accessed remotely.
Equipment
- Bruker Autoflex Speed (MALDI-TOF/TOF)
- Burker Ultraflextreme (MALDI-TOF/TOF)
- Bruker MicroTOF Q II (QqTOF)
- Bruker TIMS TOF Flex with MALDI-2 (MALDI and ESI QqTOF with ion mobility)
- JEOL Spiral TOF (MALDI-TOF/TOF)
- Masstech AP-MALDI source
- Thermo nanoHPLC with Proteineer Spotting Robot
- Thermo HPLC with Thermo QqQ MS
- Bruker Imageprep
- Vacuum Sublimation Device
- Sunchrom Matrix Sprayer
- Sunchrom Digester
- Basic histology including microtome for MSI sample prep
- Software available includes Mascot, SCILs Lab, Flex Imaging, ClinProt Tools, PEAKS Studio X, Metaboscape.
Contact
Dr Brett Hamilton
Centre for Microscopy and Microanalysis
(07) 3365 7939
b.hamilton@uq.edu.au or cmm@uq.edu.au
Level 1, Hawken Building #50, St Lucia campus