Event Details

Date:
Thursday, 13 April 2017
Time:
12:00 pm - 1:00 pm
Room:
QBI Level 7 Auditorium
UQ Location:
Queensland Brain Institute (St Lucia)
URL:
http://www.qbi.uq.edu.au/neuroscience-seminars
Event category(s):

Event Contact

Name:
Ms Deirdre Wilson
Phone:
3346 6300
Email:
d.wilson5@uq.edu.au
Org. Unit:
Queensland Brain Institute

Event Description

Full Description:
Dr Christian Winterflood, Zurich Switzerland

Title: 'Applications of supercritical angle and single-molecule fluorescence'

Abstract: Highly surface-confined and efficient fluorescence detection can be achieved by the detection of near-field mediated supercritical angle fluorescence (SAF). This approach allows for undercritical angle fluorescence to be detected from deeper axial planes simultaneously. I will present applications of SAF detection ranging from sub-diffraction imaging to biosensing. I will also present a straightforward method for two-colour 3D dSTORM. The combination of biplane imaging and spectral demixing with two highly performant dyes eliminates several problems, including crosstalk, chromatic aberration or the need for colour registration. Lastly, I will present an investigation of the diffusion barrier to the lateral exchange of molecules in the axon initial segment membrane of neurons by correlative single-particle tracking and dSTORM, revealing a highly ordered nanoscopic compartmentalisation.​​

Directions to UQ

Google Map:
Directions:
To St Lucia Campus, UQ Ipswich, and UQ Gatton.

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