QBI Seminar: 'Applications of supercritical angle and single-molecule fluorescence'

Event Details

Date:

Thursday, 13 April 2017

Time:

12:00 pm - 1:00 pm

Room:

QBI Level 7 Auditorium

UQ Location:

Queensland Brain Institute (St Lucia)

URL:

http://www.qbi.uq.edu.au/neuroscience-seminars

Event category(s):

Full Description:: Dr Christian Winterflood, Zurich Switzerland

Title: 'Applications of supercritical angle and single-molecule fluorescence'

Abstract: Highly surface-confined and efficient fluorescence detection can be achieved by the detection of near-field mediated supercritical angle fluorescence (SAF). This approach allows for undercritical angle fluorescence to be detected from deeper axial planes simultaneously. I will present applications of SAF detection ranging from sub-diffraction imaging to biosensing. I will also present a straightforward method for two-colour 3D dSTORM. The combination of biplane imaging and spectral demixing with two highly performant dyes eliminates several problems, including crosstalk, chromatic aberration or the need for colour registration. Lastly, I will present an investigation of the diffusion barrier to the lateral exchange of molecules in the axon initial segment membrane of neurons by correlative single-particle tracking and dSTORM, revealing a highly ordered nanoscopic compartmentalisation.