QBI Seminar: 'Applications of supercritical angle and single-molecule fluorescence'
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- Dr Christian Winterflood, Zurich Switzerland
Title: 'Applications of supercritical angle and single-molecule fluorescence'
Abstract: Highly surface-confined and efficient fluorescence detection can be achieved by the detection of near-field mediated supercritical angle fluorescence (SAF). This approach allows for undercritical angle fluorescence to be detected from deeper axial planes simultaneously. I will present applications of SAF detection ranging from sub-diffraction imaging to biosensing. I will also present a straightforward method for two-colour 3D dSTORM. The combination of biplane imaging and spectral demixing with two highly performant dyes eliminates several problems, including crosstalk, chromatic aberration or the need for colour registration. Lastly, I will present an investigation of the diffusion barrier to the lateral exchange of molecules in the axon initial segment membrane of neurons by correlative single-particle tracking and dSTORM, revealing a highly ordered nanoscopic compartmentalisation.
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