In vivo trials of a vaccine to reduce bovine shedding by enterohaemorrhagic E. coli
Event Details
- Date:
- Tuesday, 12 May 2015
- Time:
- 12:00 pm - 1:00 pm
- Room:
- Level 3, Large Seminar Room
- UQ Location:
- Queensland Bioscience Precinct (St Lucia)
- URL:
- http://www.qaafi.uq.edu.au/qaafi-science-seminars-2015
- Event category(s):
Event Contact
- Name:
- Mrs Luba Hickey
- Phone:
- 62092
- Email:
- l.kakoychenko@uq.edu.au
- Org. Unit:
- Queensland Alliance for Agriculture and Food Innovation
Event Description
- Full Description:
- QAAFI Science Seminar
Presented by A/Prof. Rowland Cobbold
Associate Professor in Veterinary Public Health at The University of Queensland
ABSTRACT
Cattle are the principle reservoir host for enterohaemorrhagic E. coli (EHEC), which are significant food-borne and zoonotic pathogens. O157:H7 is the archetypal strain for this E. coli pathotype. However, several other pathogenic serogroups are recognised, including O26, O111 and O103, particularly in Australia. Operational hygiene during meat processing has been the mainstay of prevention of human EHEC transmission. However, farm-level reductions in EHEC carriage will also contribute to enhanced prevention via food and via environmental pathogen contamination and direct zoonotic transmission. Of several approaches explored for reducing bovine carriage of EHEC, vaccination offers the most promise. Products currently available target E. coli O157:H7, are designed for use primarily North American beef production systems, and have other limitations that reduce their application more broadly.
The goal of the current study was to screen novel E. coli antigens for efficacy in reducing bovine carriage of non-O157 EHEC serotypes. Using a combination of bioinformatic interrogation of EHEC strains and in vitro phenotypic methods, a cluster of antigens that have potential EHEC O26 colonisation blocking effects were identified. These antigens were tested by parental immunisation of experimental cattle that were orally inoculated with EHEC O26 and observing EHEC O26 recto-anal junction (RAJ) colonisation rates for three weeks post-challenge. Intimin, a recognised effector protein for E. coli O157:H7 colonisation and pathogenesis, significantly reduced bovine RAJ colonisation by EHEC O26. Two other novel antigens were similarly associated with EHEC O26 anti-colonisation effects. Use of these antigens in a combinatorial vaccine, particularly if combined with targeted delivery systems, may offer a method for reduction of bovine-human transmission risk for non-O157 EHEC.
About A/Prof. Rowland Cobbold
A/Prof. Cobbold trained as a veterinarian and gained several years of clinical experience both in Australia and the UK before undertaking a PhD in microbiology co-jointly with the University of Queensland (UQ) and CSIRO Food Science Australia. Undertook a faculty position at Washington State University's College of Veterinary Medicine for four years, in a research-focussed position investigating food-borne disease epidemiology. Returned to Australia to a teaching and research position with the UQ School Of Veterinary Science.
Directions to UQ
- Google Map:
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- Directions:
- St Lucia Campus | Gatton campus.
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